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应用小鼠全胚胎培养技术 ,通过妊娠第 8天分别腹腔注射 5 ,10 ,15和 2 0mg·kg-1环磷酰胺 (cyclophosphamide,CP) ,研究了该药对器官原基形成期胚胎的致畸作用 ,并对其致畸机理作了初步探索。给药 4h后取胚胎进行培养 ,于第 10 5天收获胚胎 ,测量其卵黄囊直径、头长及颅臀长并记录其大体形态的变化。实验结果表明 ,15mg·kg-1剂量组引起尾畸形最为显著 ;2 0mg·kg-1剂量组生长迟缓表现较为明显。电镜观察所显示的细胞凋亡及死亡的形态学变化提示环磷酸胺致畸作用可能与其诱导的细胞凋亡和 /或死亡有关。
The whole mouse embryo culture technique was used to study the effect of the drug on the development of organ primordial embryos by intraperitoneal injection of 5, 10, 15 and 20 mg · kg -1 cyclophosphamide (CP) on day 8 of pregnancy Teratogenicity, and its teratogenic mechanism made a preliminary exploration. The embryos were harvested after 4h and the embryos were harvested on the 105th day. The diameter, head length and cranial length of the yolk sac were measured and the morphological changes were recorded. The experimental results show that 15mg · kg-1 dose group caused the most significant caudal deformity; 20mg · kg-1 dose slow growth was more obvious. Electron microscopy showed morphological changes of apoptosis and death suggest that the teratogenic effects of cyclophosphamide may be related to its induction of apoptosis and / or death.