甲基营养细菌Ｎｏ１甲胺脱氢酶是以色氨酸－色氨酰醌为辅基的一种特殊氧化还原酶．粗酶液经纯化后，其比活力和收得率分别为５．１ｎｍｏｌ·ｇ－１和２８％．该酶的分子量为６７０００，等电点为８．３和８．５，组成它的大、小两个亚基的分子量分别为３７０００和１５０００．甲胺脱氢酶有较好的耐热性，它能催化包括一级甲胺和二胺在内的底物反应，与甲胺反应的Ｋｍ值为２６．６μｍｏｌ·Ｌ－１，最适ｐＨ为８．０．其酶催化反应可被Ｃｕ２＋抑制．该酶的吸收光谱是，在３２８ｎｍ和４２６ｎｍ呈现两个特征峰 Methylotrophic bacterium No1 methylamine dehydrogenase is a special oxidoreductase that uses tryptophan-tryptamine as a prosthetic group. After the crude enzyme solution was purified, its specific activity and yield were 5.1nmol · g-1 and 28%. The enzyme has a molecular weight of 67,000 and isoelectric points of 8.3 and 8.5. The molecular weights of the two subunits, which constitute it, are 37,000 and 15,000, respectively. Methylamine dehydrogenase has good heat resistance, it can catalyze the reaction including methylamine and diamine, the reaction with methylamine Km value of 26.6μmol·L-1, the optimum pH 8.0. Its enzymatic reaction can be Cu2 + inhibition. The absorption spectrum of this enzyme shows two characteristic peaks at 328 nm and 426 nm