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利用已构建的包含287个标记位点的遗传图谱的大白菜DH群体,采用多模型QTL作图的方法,通过苗期人工接种TuMV-C4株系对来自抗病亲本Y195-93和感病亲本Y177-12的DH群体的TuMV抗性进行QTL分析。共检测到3个QTLs,分别位于R03、R04和R06连锁群上,解释的表型变异在10.5%～21.9%之间,3个QTLs可解释40.0%的表型总变异。其中位于R04上命名为Tu-2的QTL解释的表型变异最高,为21.9%,其余两个位于R03和R06上的QTLs,分别解释10.5%和14.5%的表型变异。
Using the constructed Chinese cabbage DH population with genetic map of 287 loci, using the multi-model QTL mapping method, TuMV-C4 lines were inoculated with the resistant parent Y195-93 and the susceptible parent QTL analysis of TuMV resistance in DH population of Y177-12. Three QTLs were detected in the linkage groups R03, R04 and R06, respectively. The explained phenotypic variation ranged from 10.5% to 21.9%. Three QTLs explained 40.0% of total phenotypic variation. The QTLs named Tu-2 at R04 explained the highest phenotypic variation at 21.9%. The remaining two QTLs at R03 and R06 explained 10.5% and 14.5% of the phenotypic variation, respectively.