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用6对INDEL标记及10对SSR标记对74份黑龙江及吉林的粳稻资源材料进行检测,通过分子标记构建每份材料的指纹图谱,利用Structure及Powermarker V3.25软件对供试材料进行分群及聚类分析。结果表明参试材料可以分成两个大群,细分为4个小群,在聚类分析图中,黑龙江和吉林品种中的龙粳30、垦稻20、通823等品种与籼型对照品种9311及偏籼型材料C418聚在一群,其余的粳稻品种分布在不同的3个小群,3个小群中的粳稻材料遗传差异较小,亲缘关系较近,试验结果与田间表现及测定结果相一致,在实际育种中,可以参照聚类图中信息,选择亲缘关系稍远的偏籼型材料进行杂交配组。
Sixty INDEL markers and ten pairs of SSR markers were used to detect 74 japonica rice resources in Heilongjiang and Jilin provinces. The fingerprint of each material was constructed by molecular markers. The structure and Powermarker V3.25 software were used to cluster and poly Class analysis The results showed that the tested materials could be divided into two large groups, which were subdivided into four subgroups. Among the cluster analysis plots, Longjing 30, Keng 20, Tong 823 and Heilongjiang and Jilin varieties 9313 And the indica type material C418 clustered in one group. The rest of japonica rice varieties distributed in three different subgroups. The genetic diversity of japonica rice among the three subgroups was small and the genetic relationship was relatively close. The results were in good agreement with the field performance and the determination results Consistent, in the actual breeding, you can refer to the information in the cluster diagram, select the genetic relationship between distant indica-type materials for hybridization.