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采集落叶松八齿小蠹成虫、幼虫、蛹,以及母坑道、子坑道、蛹室的韧皮组织和蓝变组织。采用马丁氏培养基和PDA综合培养基对各虫态体表、体内、坑道内真菌进行分离培养,统计真菌检出率。对获得的真菌菌株通过形态学和分子生物学相结合的方法进行鉴定。在落叶松八齿小蠹虫体内外和坑道内共分离出真菌31种,其中接合菌3种,子囊菌8种,担子菌1种,半知菌19种。自各虫态体表共分离出真菌23种,体内共分离出真菌8种,31种真菌在坑道内均有检出。其中,Ceratocystis fujiensis为中国新记录种,Ophiostoma sp.有待进一步鉴定,二者为在多数分离部位检出、且检出率较高的蓝变真菌,与蠹害的发生存在一定的关系。
The adults, larvae and pupae of Larix principis-rupprechtii were collected, and the phloem tissues and blue-transformed tissues of the main tunnel, sub-tunnel and pupal chamber were collected. Using Marten’s medium and PDA comprehensive medium, the fungi in body surface, body and tunnel of each worm body were isolated and cultured, and the detection rate of fungi was counted. The fungal strains obtained were identified by a combination of morphological and molecular biology. 31 species of fungi were isolated from the inside and outside of the larch, Bethoda bark beetle and in the tunnel, including 3 kinds of conjugate bacteria, 8 kinds of ascomycetes, 1 kinds of basidiomycetes and 19 kinds of semi-know bacteria. Twenty three fungi were isolated from the body surface of each worm body, eight fungi were isolated from the body, and 31 fungi were detected in the tunnel. Among them, Ceratocystis fujiensis is a new recorded species in China. Ophiostoma sp. Needs to be further identified. Both of them are blue-change fungi that are detected at most isolated sites and have a high detection rate, which is related to the occurrence of pests.