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目的 研制用于疟疾简便、快速诊断试剂盒的试剂。方法 利用噬菌体抗体库技术，构建抗恶性疟原虫丝氨酸重复抗原（ＳＥＲＡ）的噬菌体抗体库。经３轮“吸附－洗脱－扩增”的富集反应后，从中筛选抗ＳＥＲＡ的阳性克隆株，并进行可溶性诱导表达，最后用ＥＬＩＳＡ和Ｗｅｓｔｅｒｎ ｂｌｏｔ等进行鉴定。结果 成功地构建了中等库容的噬菌体抗体库，并从中筛选到９株抗ＳＥＲＡ的阳性克隆株，表达的单链抗体的相对分子质量大小约为３１ｋＤａ，能与 ＳＥ４７＇抗原起特异性结合反应。结论 成功地构建了抗ＳＥ４７＇的噬菌体抗体库，从中筛选制备的单链抗体为研制疟疾快速诊断试剂盒奠定了基础。
Objective To develop a reagent for the simple and rapid diagnosis of malaria. Methods The phage antibody library of anti-Plasmodium falciparum serine repeat antigen (SERA) was constructed by using phage antibody library technology. After 3 rounds of “adsorption-elution-amplification” enrichment reaction, the positive clones against SERA were screened and induced by soluble expression, and finally identified by ELISA and Western blot. Results The phage antibody library was successfully constructed and 9 positive clones against SERA were screened out. The relative molecular mass of the expressed single chain antibody was about 31 kDa, which could specifically bind to SE47 ’antigen. Conclusion The phage antibody library against SE47 ’was constructed successfully. The single-chain antibody screened from it has laid a foundation for the rapid diagnosis of malaria.