目的 :克隆和研究高低分化胆管癌间差异表达基因。方法 :对mRNA差异显示PCR(DDRT PCR)的一系列条件进行了探索和改进 ,建立了有效的DDRT PCR法。结果 :在高、低分化胆管癌差异分化基因的研究方面获得了满意的结果 ,共获得 9个有显著差异的cDNA片段 ,测序后进行同源序列比较 ,有一个与层粘连蛋白受体基因高度同源 ,还有一个可能是一个新基因的部分序列。结论 :通过对传统方法的改进 ,大大提高了工作效率 ,降低了实验成本和工作量并对相关问题进行了探讨 ,为进一步的研究奠定了基础。 OBJECTIVE: To clone and study differentially expressed genes between high and low differentiated cholangiocarcinomas. Methods: A series of conditions of mRNA differential display PCR (DDRT PCR) were explored and improved, and an effective DDRT PCR method was established. Results: Satisfactory results were obtained in the study of differentially differentiated genes in high and poorly differentiated cholangiocarcinoma. Nine cDNA fragments with significant differences were obtained. After sequencing, homologous sequences were compared. There was a significant difference Homologous, there is a possible part of a new gene sequence. Conclusion: Through the improvement of the traditional methods, the work efficiency is greatly improved, the experimental cost and workload are reduced, and the related problems are discussed, which lays the foundation for further research.