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促分裂原活化蛋白激酶(mitogen-activated protein kinases,MAPK)在脱落酸(abscisic acid,ABA)诱导活性氧(reactive oxygen species,ROS)产生中起重要作用,然而MAPK家族相关基因在花芽休眠解除过程中的表达变化尚不清楚。本研究以六年生‘中油四号’油桃花芽为材料,利用NCBI找出桃树上的MAPK同源基因,测定了MAPK家族相关基因的组织表达特性及休眠解除过程中在花芽中的基因表达水平。结果表明:桃中有15个MAPKs,通过系统进化分析分为A、B、C、D四个组;15个MAPKs基因在花芽、根、枝、叶、花、果实、种子中均有表达,其中Pp MPK2在种子中表达量最高,其次是花芽,在其他组织中表达量较低,PpMPK11在花中表达量较高,在其他组织中均较低;MAPKs基因中6个MAPKs在花芽休眠解除过程中呈显著的表达下调,与休眠解除的进程一致。我们推测有MAPKs基因可能参与花芽休眠解除的调控。
Mitogen-activated protein kinases (MAPK) play an important role in abscisic acid (ABA) -induced reactive oxygen species (ROS) production. However, MAPK-related genes play an important role in flower bud dormancy release The change in expression is not clear. In this study, we used NCBI to identify the MAPK homologous genes of peach trees from the perennial ’Zhongyou No.4’ nectarine flower buds and determined the tissue expression characteristics of MAPK family related genes and the gene expression in floral buds during dormancy release Level. The results showed that there were 15 MAPKs in peach and divided into four groups A, B, C and D through phylogenetic analysis. Fifteen MAPKs genes were expressed in flower buds, roots, branches, leaves, flowers, fruits and seeds, Among them, Pp MPK2 had the highest expression level in seeds, followed by flower buds, and lower expression in other tissues. The expression level of PpMPK11 in flowers was high, and it was lower in other tissues. Six MAPKs MAPKs were released in flower bud dormancy The process was significantly decreased expression, consistent with the process of dormancy release. We speculate that MAPKs may be involved in the regulation of flower bud dormancy release.