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目的:观察蛇黄肝炎合剂在乙型肝炎病毒(HBV)基因转染的人肝癌细胞系2215细胞培养中,对细胞分泌的表面抗原(HBsAg)及e抗原(HBeAg)的抑制作用。方法:接种2215细胞24h后,加入不同浓度的药物培养5天,观察药物对细胞的毒性。在无毒质量浓度下,将药物加入2215细胞培养5天,测定培养液中HBsAg和HBeAg水平。结果:蛇黄肝炎合剂在1∶16倍稀释时对细胞的破坏率为63.49%,1∶32倍稀释浓度时对细胞破坏率<50%,在无毒浓度1∶32倍稀释下能抑制细胞分泌HBsAg达64.83%,抑制细胞分泌HBeAg达86.1%。结论:蛇黄肝炎合剂对2215细胞分泌HBsAg和HBeAg具有明显的抑制作用。
OBJECTIVE: To observe the inhibitory effect of snake liver mixture on surface antigen (HBsAg) and e antigen (HBeAg) secreted by human hepatoma cell line 2215 transfected with Hepatitis B virus (HBV) gene. Methods: After inoculation of 2215 cells for 24 hours, different concentrations of drugs were added and cultured for 5 days to observe the toxicity of the drugs on the cells. In non-toxic mass concentration, the drug was added to the 2215 cell culture for 5 days to determine the level of HBsAg and HBeAg in the culture solution. Results: The rate of cell destruction was 63.49% when diluted with 1:16 dilution. The rate of cell destruction was less than 50% when 1:32 dilution, and the cells were inhibited by 1:32 dilution Secretion of HBsAg up to 64.83%, inhibition of cell secretion of HBeAg up to 86.1%. Conclusion: Snake Huangheyan mixture can significantly inhibit the secretion of HBsAg and HBeAg in 2215 cells.