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本研究按不同给药剂量、不同给药时间，以醋酸地塞米松（Ｄｅｘａｍｅｔｈａｓｏｎａｃｅｔａｔｅ，ＤＡ）为致畸药物，诱发远交系ＮＩＨ小白鼠胚胎腭裂，成功地建立了可供各种实验研究的腭裂动物模型。经过统计学处理以及光镜、扫描电镜观察，结果表明：①妊娠第１２天为最佳致畸时间；②５０ｍｇ／ｋｇＤＡ为最佳致畸量；③妊娠第１０～１２天每天注射５０ｍｇ／ｋｇＤＡ可使ＮＩＨ胚鼠１００％致畸；④致畸量与致畸程度和致畸率呈正相关性，其相关系数分别为０．９７９１和０．９９９８。本文还就糖皮质激素致腭裂畸形的机理等问题进行了讨论。
In this study, dexamethasone acetate (DA) was used as teratogenic agent to induce embryonic cleft palate of NIH mice at different doses and times of administration, and cleft palate was successfully established for various experimental studies Animal model After statistical analysis and light microscopy, scanning electron microscopy, the results showed that: ① the best teratogenic time on the twelfth day of pregnancy; ② 50mg / kgDA for the best teratogenicity; ③ pregnant day 10 to 12 days daily injection of 50mg / kgDA So that NIH embryos 100% teratogenic; ④ teratogenicity and teratogenicity and teratogenic rates were positively correlated, the correlation coefficients were 0.9791 and 0.9998. This article also discussed the mechanism of glucocorticoid induced cleft palate deformities and other issues were discussed.