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目的检测乳腺癌中DNA结合抑制因子4(ID4)基因启动子区甲基化水平及其表达。方法采用焦磷酸测序法定量检测乳腺癌(A组,40例)及正常乳腺组织(B组,20例)标本中ID4基因启动子区甲基化水平,分析其与临床病理特征间的相关性;免疫组织化学SP法检测组织标本中ID4的表达。结果 A组ID4启动子区甲基化水平为(31.16±1.5)%,高于B组的(19.89±0.22)%(P<0.01);雌激素受体(ER)阳性乳腺肿瘤组织中ID4甲基化水平为(36.57±1.97)%,高于ER阴性组织的(27.91±1.83)%(P<0.01)。在乳腺癌组织和正常组织中,ID4的蛋白表达水平与其启动子甲基化水平呈负相关(r=-0.478,P<0.01)。结论 ID4基因在乳腺癌中呈高甲基化状态(特别在ER阳性的乳腺癌中);ID4基因启动子区甲基化可能是乳腺癌的发病机制之一。
Objective To detect the methylation level of DNA binding inhibitor 4 (ID4) gene in breast cancer and its expression. Methods The methylation level of ID4 gene in breast cancer (group A, 40 cases) and normal breast tissue (group B, 20 cases) was quantitatively detected by pyrosequencing method. The correlation between the methylation status and clinicopathological features was analyzed Immunohistochemistry was used to detect the expression of ID4 in tissue samples. Results The methylation level of ID4 promoter in group A was (31.16 ± 1.5)%, which was significantly higher than that in group B (19.89 ± 0.22)% (P <0.01). ID4 methylation in estrogen receptor (ER) positive breast cancer The level of methylation was (36.57 ± 1.97)%, higher than that of ER negative (27.91 ± 1.83)% (P <0.01). In breast cancer tissues and normal tissues, the protein expression level of ID4 was negatively correlated with its promoter methylation level (r = -0.478, P <0.01). Conclusion ID4 gene is hypermethylated in breast cancer (especially in ER-positive breast cancer). Methylation of ID4 gene promoter may be one of the pathogenesis of breast cancer.