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乙二醇甲基丙烯酸酯(GMA)作为光学显微镜标本的包埋剂可进行形态学观察,同时很好地保存了酶的活性。我们应用改进的GMA低温真空包埋技术对不脱钙的小鼠骨髓作连续切片进行酶组织化学染色取得了令人满意的结果。
Ethylene glycol methacrylate (GMA) as an optical microscope specimen embedding agent for morphological observation, while well preserved the activity of the enzyme. We applied the improved GMA cryogenic vacuum embedding technique to do serial sections of non-decalcified mouse bone marrow for enzymatic histochemical staining with satisfactory results.