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目的探究小干扰RNA对人卵巢癌细胞株CXCR4基因表达和侵袭力的影响。方法利用单细胞克隆技术从人卵巢癌细胞系SW626中分离培养细胞株CXCR4,并设计合成针对CXCR4基因的小干扰RNA,在脂质体介导下,重组表达质粒转染人卵巢癌细胞株,利用RT-PCR方法对CXCR4基因表达进行检查,观察转染后细胞株的侵袭能力。实验细胞分为不做处理的空白对照组、转染空病毒载体的阴性对照组、siRNA组。结果 siRNA组的CXCR4 CXCR4 mRNA水平、蛋白表达率、穿膜细胞数均明显低于空白对照组和阴性对照组(P<0.05),而空白对照组与阴性对照组之间无明显差异(P>0.05)。结论小干扰RNA可有效抑制人卵巢癌细胞株CXCR4基因表达和侵袭力。
Objective To investigate the effect of small interfering RNA on CXCR4 gene expression and invasiveness in human ovarian cancer cell lines. Methods The cell line CXCR4 was isolated and cultured from human ovarian cancer cell line SW626 by single cell cloning technique. Small interfering RNA targeting CXCR4 gene was designed and synthesized. The recombinant plasmid was transfected into human ovarian cancer cell line with lipofectamine. The expression of CXCR4 gene was examined by RT-PCR and the invasion ability of the transfected cells was observed. The experimental cells were divided into blank control group without treatment, negative control group transfected with empty virus vector and siRNA group. Results The mRNA expression of CXCR4, the expression of CXCR4 and the number of transmembrane cells in the siRNA group were significantly lower than those in the blank control group and the negative control group (P <0.05), but there was no significant difference between the blank control group and the negative control group (P> 0.05). Conclusion Small interfering RNA can effectively inhibit CXCR4 gene expression and invasiveness in human ovarian cancer cell lines.