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目的:建立快速测定甘草及甘草浸膏中甘草酸和甘草苷含量的超高效液相色谱(UPLC)方法。方法:采用超高效液相色谱仪(UPLC),在BEH Shield RP18(2.1 mm×50 mm,1.7μm)色谱柱上梯度洗脱分离,流动相乙腈(A),1.0%乙酸水(B),流速0.5 mL·min-1,甘草苷检测波长276 nm,甘草酸检测波长254 nm,柱温30℃。结果:甘草苷的浓度在0.01～1.0 g·L-1与峰面积线性关系良好,平均回收率为98.98%,方法精密度RSD 2.55%(n=6),甘草酸在0.03～1.2 g·L-1与峰面积线性关系良好,平均回收率为99.03%,方法精密度RSD 1.73%(n=6)。结论:甘草苷和甘草酸在7 min内获得良好分离,结果准确可靠,该方法可用于甘草及其浸膏中甘草酸和甘草苷的快速测定。
OBJECTIVE: To establish a rapid ultra performance liquid chromatography (UPLC) method for the determination of glycyrrhizin and glycyrrhizin in licorice and licorice extract. METHODS: The residue was separated on a BEH Shield RP18 (2.1 mm × 50 mm, 1.7 μm) column by ultra performance liquid chromatography (UPLC). The mobile phase consisted of acetonitrile, 1.0% acetic acid and water (B) The flow rate was 0.5 mL · min-1, the detection wavelength of glycyrrhizin was 276 nm, the detection wavelength of glycyrrhizin was 254 nm, and the column temperature was 30 ℃. Results: The linear range of the concentration of glycyrrhizin was between 0.01 and 1.0 g · L-1 and the peak area was linear. The average recovery was 98.98%. The method precision was 2.55% RSD (n = 6) and glycyrrhizin was 0.03-1.2 g · L The linearity of peak area was linear with the average recovery of 99.03%. The precision of the method was 1.73% (n = 6). CONCLUSION: Glycyrrhizin and glycyrrhizic acid are well separated within 7 min, and the results are accurate and reliable. The method can be used for the rapid determination of glycyrrhizin and glycyrrhizin in licorice and its extract.