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[Objective] To make the use of high performance liquid chromatographic method (HPLC) to determine the content of active ingredient adenosine in RHIZOMA ET RADIX BAPHICACANTHIS CUSIAE from different production places in Guangdong Province. [Method] A HPLC method was established for determining the content of adenosine. To be specific, HPLC analysis was performed on a chromatographic column ZORBAXExtend-C18 (4.6 mm×250 mm, 5 μm) with methanol-water(V/V, 7∶93)as the mobile phase, detection wavelength at 260 nm, flow rate at 1.0 ml/min and column temperature at 30 ℃. [Result] Adenosine had good linear relationship between concentration and peak area from 4.65 to 232.6 ng/ml, the recovery rate was 100.3%, RSD=2.2%(n=6); the sample from Conghua in Guangdong Province had the highest content, while the two wild species had the lowest of that. [Conclusion] The method is simple, accurate, rapid and reproducible. It can complete the existing quality standard of RHIZOMA ET RADIX BAPHICACANTHIS CUSIAE compound feed additives, and make the control of quality efficiently.
[Objective] To make the use of high performance liquid chromatographic method (HPLC) to determine the content of active ingredient adenosine in RHIZOMA ET RADIX BAPHICACANTHIS CUSIAE from different production places in Guangdong Province. [Method] A HPLC method was established for determining the content of adenosine. To be specific, HPLC analysis was performed on a chromatographic column ZORBAXExtend-C18 (4.6 mm × 250 mm, 5 μm) with methanol-water (V / V, 7:93) as the mobile phase, detection wavelength at 260 [Result] Adenosine had good linear relationship between concentration and peak area from 4.65 to 232.6 ng / ml, the recovery rate was 100.3%, RSD = 2.2% (n = 6); the sample from Conghua in Guangdong Province had the highest content, while the two wild species had the lowest of that. [Conclusion] The method is simple, accurate, rapid and reproducible. It can complete the existing quality standard of RHIZOMA ET RADIX BAPHICACANTH IS CUSIAE compound feed additives, and make the control of quality efficiently.