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目的探讨CCR2基因-190G/A和CCR5基因-59029G/A单核苷酸多态性(SNP)与结核性脓胸的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对200例结核性脓胸患者和200例健康人进行-190G/A和-59029G/A SNPs检测,采用逐步logistic回归分析其与结核性脓胸的相关性。结果与CCR2基因-190G/G基因型相比,携带A/G和A/A基因型均可增加结核性脓胸发病风险(OR=2.250,95%CI=1.046~4.842,P=0.037和OR=8.706,95%CI=4.232~17.909,P=0.000)。无卡介苗接种史亦增加结核性脓胸发病风险(OR=3.031,95%CI=2.109~4.356,P=0.000)。CCR5基因-59029G/A位点各基因型未进入回归方程,与结核性脓胸的发病风险无明显相关性。结论 CCR2基因-190G/A SNP与结核性脓胸有相关性,CCR5基因-59029G/A SNP可能与结核性脓胸无关。
Objective To investigate the association between -59029G / A single nucleotide polymorphism (SNP) of CCR2 gene-190G / A and CCR5 gene and tuberculous empyema. Methods PCR-RFLP was used to detect -190G / A and -59029G / A SNPs in 200 cases of tuberculous empyema and 200 healthy controls. Logistic regression Analysis of its correlation with tuberculous empyema. Results Compared with CCR2 gene-190G / G genotype, both A / G and A / A genotypes increased the risk of tuberculous empyema (OR = 2.250, 95% CI = 1.046-4.842, P = 0.037 and OR = 8.706, 95% CI = 4.232-17.909, P = 0.000). No history of BCG vaccination also increased the risk of tuberculous empyema (OR = 3.031, 95% CI = 2.109 ~ 4.356, P = 0.000). CCR5 -59029G / A locus genotypes did not enter the regression equation, and the incidence of tuberculous empyema no significant correlation. Conclusion CCR2 gene-190G / A SNP is associated with tuberculous empyema, CCR5 gene-5909G / A SNP may be unrelated to tuberculous empyema.