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将嗜水气单胞菌(Aeromonashydrophila ,Ah)J 1菌株第 5代菌种通过鲫鱼体内复壮后 ,制备基础种子。将基础种子在普通营养琼脂培养基上连续传至 10代 ,分别测第 1代和第 10代的培养特性、免疫原性以及其毒力 ;同时 ,对Ah菌种的保存条件进行了优化。结果嗜水气单胞菌菌种在 1～ 10代之内 ,其培养特性、免疫原性以及毒力完全相同 ,表明嗜水气单胞菌基础种子的使用限定代次至少可达 10代。Ah菌种以 15 %甘油肉汤、 - 2 0℃保存为佳 ,保存期可达两年。
The fifth generation of Aeromonas hydrophila (Ah) J 1 strain was rejuvenated in vivo by crucian carp to prepare basic seeds. The basic seeds were propagated continuously on common nutrient agar medium for 10 generations, and the culture characteristics, immunogenicity and virulence of 1st and 10th passages were measured respectively. Meanwhile, the preservation conditions of Ah strains were optimized. Results Aeromonas hydrophila strains within 1 to 10 generations, the culture characteristics, immunogenicity and virulence exactly the same, indicating that the use of Aeromonas hydrophila basic seed limited generations of at least up to 10 generations. Ah strain with 15% glycerol broth, -20 ℃ preservation better, the shelf life of up to two years.