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以脱氧雪腐镰刀菌烯醇(Deoxynivalenol,DON)及其代谢产物脱环氧DON(DeepDON)为检测对象,建立了一种同时检测猪盲肠内容物中2种毒素的高效液相色谱分析方法。样品经乙腈提取后,分别采用吸附剂:木炭粉末+氧化铝+硅藻土(7+5+3,w+w+w)和Bond Elut Mycotoxin多功能净化柱对DON和DeepDON进行净化,HPLC检测,采用C18色谱柱,乙腈/甲醇/水(5/5/90,v/v/v)为流动相,流速1.0mL/min,检测波长220nm。结果显示DON和DeepDON在0.5～10μg/mL范围内线性关系良好(R2=0.9999,0.9998)。
A high performance liquid chromatography (HPLC) method for the simultaneous determination of two toxins in porcine caecal contents was established using deoxynivalenol (DON) and its metabolite DON-DeepDON as test objects. After the sample was extracted with acetonitrile, DON and DeepDON were purified by adsorbent: charcoal powder + alumina + diatomite (7 + 5 + 3, w + w + w) and Bond Elut Mycotoxin multifunctional purifying column respectively. The mobile phase consisted of C18 column, acetonitrile / methanol / water (5/5/90, v / v / v), flow rate 1.0 mL / min and detection wavelength 220 nm. The results showed that there was a good linear relationship between DON and DeepDON in the range of 0.5 ~ 10μg / mL (R2 = 0.9999,0.9998).