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为了扩大生产肝素的原料,挖掘具有特殊药理作用的肝素类药物,立足实际生产情况,参照盐解和酶解两种工艺,以牛,羊、猪肠粘膜和牛、羊肺为原料,制备了五种肝素,并对其进行了理化分析和初步的药理作用研究。材料和方法材料: Triton WR—1339,Serva,用生理盐水配成10％的溶液,肝素钠(国家标准品),168u/mg,北京药品生物制品检定所;其它试剂均为分析纯或化学纯。日立282型分析超速离心机;UV-260紫外-可见分光光度计;PEM783型红外分光光度计。
In order to expand the production of heparin raw materials, excavation of heparin with special pharmacological effects, based on the actual production, with reference to salt hydrolysis and enzymatic hydrolysis of two processes, cattle, sheep, pig intestine and cattle, sheep lung as raw material, prepared five Heparin, and its physical and chemical analysis and preliminary pharmacological effects. MATERIALS AND METHODS MATERIALS: Triton WR-1339, Serva, 10% solution in physiological saline, heparin sodium (national standard), 168u / mg, Beijing Institute for the Control of Pharmaceutical and Biological Products; all other reagents were of analytical or chemical purity . Hitachi 282-type analytical ultracentrifugation; UV-260 UV - visible spectrophotometer; PEM783-type infrared spectrophotometer.