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目的 观察化瘀通阳方对溃疡性结肠炎(UC)大鼠巨噬细胞炎性蛋白-2(MIP-2)的影响.方法 将54只Wister大鼠随机取14只为空白对照组,其余40只为模型组,采用葡聚糖硫酸钠(DSS)自由饮用结合灌胃方法进行模型复制,将模型组随机分为生理盐水组、美沙拉嗪组、化瘀通阳组,每组10只,分别给予生理盐水、美沙拉嗪灌肠液、化瘀通阳中药进行灌肠治疗,给药10天后剖取大鼠结肠黏膜组织.用生物素标记各组黏膜组织的总蛋白,采用细胞因子抗体芯片分析各组的差异表达蛋白.对筛选出的蛋白采用免疫组化方法进行验证.结果 芯片分析筛选出模型组中MlP-2表达较空白对照组有显著差异,美沙拉嗪组、化瘀通阳组较模型组为低表达.免疫组化验证空白对照组未见或见少量棕色染色的MIP-2蛋白表达,模型组染色程度高于空白对照组,评分比较差异有统计学意义(P＜0.05),美沙拉嗪组、化瘀通阳组与生理盐水组比较,染色程度低于生理盐水组(P＜0.05).结论 UC大鼠活动期MIP-2表达明显升高;化瘀通阳方与美沙拉嗪皆能够降低MIP-2的表达.“,”Objective To observe the effect of Huayu Tongyang Recipe (HTR) on macrophage inflammatory protein-2 (MIP-2) in rats with ulcerative colitis (UC).Methods Totally 54 Wistar rats were randomly divided into the blank control group (n =14) and the model group (n =40).UC rat model was duplicated by free drinking 5％dextran sulphate sodium (DSS) solution and gastric administration of DSS solution.After modeling,successfully 30 modeled rats in the model group were randomly divided into normal saline group,mesalazine group,HTR group,10 rats in each group.Enema treatment was given to rats in normal saline,mesalazine enema,and HTR groups respectively.After 10 days medication,the colonic mucosa of rats were dissected.The total protein of the mucosal tissues of each group was labeled with biotin.The differentially expressed proteins were analyzed by using cytokine antibody microarray.The identified proteins were tested by immunohistochemistry.Results The expression of MIP-2 in the model group was significantly different from that in the blank control group.Compared with the model group,the expression of MIP-2 was lower in mesalazine group and HYR group after treatment.Immunohistochemistry showed that there was no or a small amount of brown stained MIP-2 expression in the blank control group.The staining degree of the model group was higher than that of the blank controlgroup,with statistical difference (P ＜0.05).Compared with the normal saline group,the staining degree was lower in mesalazine group and HTR group (P ＜0.05).Conclusions The activity of MIP-2 expression increased significantly in active stage UC rats.HYR and mesalazine could effectively reduce the expression of MIP-2.