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目的观察缬沙坦对高糖环境下大鼠肾小球系膜细胞氧化应激水平的影响。方法高糖(20 mmol.L-1)体外培养大鼠肾小球系膜细胞,不同浓度缬沙坦(10-7,10-6,10-5和10-4mol.L-1)干预,以RT-PCR技术检测细胞内还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶亚基p22phox的mRNA表达的变化,并以DCFH-DA和HE标记细胞内活性氧(ROS),流式细胞术检测细胞内ROS相对浓度。结果高糖时间相关性诱导系膜细胞超氧阴离子(.O2)与过氧化氢(H2O2)明显升高,并引起p22phox的mRNA表达显著上调,缬沙坦对高糖引起系膜细胞p22phox的mRNA表达增加有下调作用,明显降低细胞内.O2和H2O2浓度,并呈浓度依赖性。结论缬沙坦抑制高糖引起肾小球系膜细胞氧化应激可能和下调p22phox的mRNA表达有关。
Objective To observe the effect of valsartan on oxidative stress in rat mesangial cells under high glucose condition. Methods Rat mesangial cells were cultured in vitro with high glucose (20 mmol·L-1). Valsartan (10-7, 10-6, 10-5 and 10-4 mol·L-1) The mRNA expression of intracellular reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunit p22phox was detected by RT-PCR. The intracellular reactive oxygen species (ROS) were labeled with DCFH-DA and HE Cytometry was used to detect the relative concentration of intracellular ROS. Results High glucose induced a significant increase of superoxide anion (.O2) and hydrogen peroxide (H2O2) in mesangial cells and caused a significant up-regulation of p22phox mRNA expression. Valsartan induced mesangial cell p22phox mRNA Increased expression of a down-regulation effect, significantly reduce intracellular O2 and H2O2 concentration, and a concentration-dependent manner. Conclusion Valsartan inhibits high glucose induced oxidative stress in glomerular mesangial cells may be related to the down-regulation of p22phox mRNA expression.