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以国家果树种质兴城梨、苹果圃保存的314份来自19个国家的苹果栽培品种为试材,利用TP-M13-SSR标记,基于TP-M13-SSR指纹图谱构建苹果种质分子身份证。16对SSR引物在供试种质间共检测出等位基因357个,平均每对引物检测到22.3个。根据引物扩增等位基因数和Shannon’s指数,从1对引物开始逐步增加引物数量筛选可将供试材料全部区分的引物组合,最终确定6对核心引物可以区分全部供试苹果种质。基于供试苹果种质在6个SSR位点的指纹图谱,将等位基因编码成字符串获得分子身份证,利用条码技术其进一步转化成可被机器快速扫描的条码分子身份证,使每份种质具有可辨的分子身份证,达到利用最少、最特异引物区分最多苹果种质的目的。
Based on the TP-M13-SSR fingerprinting, 314 apple cultivars from 19 countries preserved in Xingguo Pear and apple garden of national fruit tree were used as materials to construct apple germplasm molecular ID card . A total of 16 pairs of SSR primers detected 357 alleles in the tested germplasm, with an average of 22.3 per primer pair. According to the number of alleles amplified by primers and Shannon’s index, the number of primers was gradually increased from one pair of primers to select the primer combinations that could completely distinguish the tested materials. Finally, six pairs of core primers could distinguish all the tested apple germplasms. Based on the fingerprinting of the tested apple germplasm at the six SSR loci, the alleles were encoded into strings to obtain molecular identification cards that were further transformed into barcode molecular identification cards that were quickly scanned by the machine using barcode technology so that each Germplasm with a distinguishable molecular ID card, to use the least, the most specific primer to distinguish the purpose of the largest apple germplasm.