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目的分析使用快速C反应蛋白(CRP)测定仪时,在全血模式下,红细胞比容(HCT)对结果的影响,并初步探讨校正方案。方法 (1)使用乙二胺四乙酸(EDTA-K2)抗凝的全血,配制两组HCT系列梯度的标本,用快速CRP测定仪测定其CRP水平,同时用血细胞分析仪测定其HCT,探讨HCT对全血CRP水平的影响;(2)检测120例患者的血液标本,每例患者同时测定其HCT、快速法测定全血CRP,IMMAGE 800测定血清CRP(作为参照检测系统),以后者为因变量、前两者为自变量做线性回归,推导校正公式;(3)使用50例HCT处于不同范围的标本验证校正公式。结果 (1)全血CRP水平与HCT值呈显著负相关关系(P<0.05);(2)以120例标本的全血CRP水平和HCT值推导出的校正公式为:CRP校正=49.3×HCT+0.84×CRP全血-14.17(R2=0.991,P<0.05);(3)Bland-Altman分析法显示,校正前全血CRP水平与参照结果无一致性,而校正后则一致性很好。结论 HCT值显著地影响全血CRP测定结果,有必要对全血模式下测定的CRP结果结合HCT进行校正,以使结果更准确。
Objective To analyze the effect of hematocrit (HCT) on the outcome of whole blood model when using the rapid C-reactive protein (CRP) detector and to investigate the calibration scheme. Methods (1) The whole blood of EDTA-K2 anticoagulation was used to prepare two sets of HCT series gradient specimens. CRP levels were measured by rapid CRP analyzer, and HCT was measured by hematology analyzer HCT on the level of CRP in whole blood; (2) Blood samples of 120 patients were detected, HCT of each patient was measured at the same time, whole blood CRP was measured by rapid method, and serum CRP was measured by IMMAGE 800 (as reference system) Dependent variables, the first two linear regression as independent variables, derived correction formula; (3) the use of 50 cases of HCT in different areas of the sample verification and correction formula. Results (1) There was a significant negative correlation between CRP level and HCT in whole blood (P <0.05). (2) The calibration formula of CRP level and HCT value in 120 cases was: CRP correction = 49.3 × HCT + 0.84 × CRP whole blood -14.17 (R2 = 0.991, P <0.05). (3) The Bland-Altman analysis showed that the level of CRP in pre-calibration whole blood was not consistent with the reference results, but the corrected consistency was good. Conclusions The HCT value significantly affects the CRP determination of whole blood. It is necessary to calibrate the CRP assay combined with HCT in whole blood mode to make the result more accurate.