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以多枝赖草种子为材料,在MS+3mg/L2,4-D+0.5mg/L激动素+3%蔗糖+0.5%琼脂培养基上暗培养,产生愈伤组织。愈伤组织经多次继代培养后仍可保持分化潜力。在1/2MS+0.5mg/L6-BA+0.5mg/LIBA+3%蔗糖+0.5%琼脂培养基上光照培养后分化出丛生状绿芽,并大量生根,形成再生植株。
Using the seeds of Leymus secalis, callus was cultured in the medium of MS + 3mg / L2,4-D + 0.5mg / L kinetin + 3% sucrose + 0.5% agar medium to produce callus. After several subcultures, the callus can still maintain its differentiation potential. The shoots of green shoots were differentiated from 1 / 2MS + 0.5mg / L 6-BA + 0.5mg / L IBA + 3% sucrose + 0.5% agar medium and rooted in large numbers to form regenerated plants.