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目的探讨白细胞介素10(IL-10)对重症急性胰腺炎(SAP)大鼠肾功能障碍的保护作用。方法将SD大鼠54只随机分为3组,假手术组(SO)、SAP组、SAP加IL-10治疗组(SAP+IL- 10),以5%牛磺脱氧胆酸钠逆行胰胆管注射建立SAP模型,观察各组术后12、24 h血清淀粉酶,肿瘤坏死因子α(TNF-a)、1L-6、尿素氮(BUN)、肌酐(Cr)水平,以及腹水量、胰腺和肾脏的病理改变,逆转录-聚合酶链反应(RT-PCR)分析肾脏细胞间黏附分子1(ICAM一1)mRNA表达水平。结果SAP组制模后血清TNF-α、IL-6水平明显上升,肾脏ICAM-1 mRNA表达上调(P<0.01),BUN、Cr在12、24 h持续升高。与SAP组比较,SAP+IL-10组血清TNF-α、IL-6、BUN、Cr水平明显下降,ICAM-1 mRNA表达下调(P<0.01),肾脏病理改变得到改善。结论IL-10可抑制TNF-α、IL-6血清水平,下调肾脏ICAM-1基因表达,改善SAP肾功能障碍。
Objective To investigate the protective effect of interleukin 10 (IL-10) on renal dysfunction in rats with severe acute pancreatitis (SAP). Methods Fifty-four SD rats were randomly divided into three groups: sham operation group (SO), SAP group, SAP plus IL-10 treatment group (SAP + IL-10) and 5% sodium taurodeoxycholate retrograde cholangiopancreatic duct The SAP model was established by injection. The levels of serum amylase, tumor necrosis factor alpha (TNF-a), 1L-6, BUN and creatinine were measured at 12 and 24 hours after operation. The pathological changes of kidney and ICAM-1 mRNA expression were analyzed by reverse transcription-polymerase chain reaction (RT-PCR). Results The levels of serum TNF-α and IL-6 in SAP group were significantly increased (P <0.01), and the levels of BUN and Cr in the SAP group increased continuously at 12 and 24 hours. Compared with SAP group, the level of serum TNF-α, IL-6, BUN and Cr in SAP + IL-10 group was significantly decreased, while the expression of ICAM-1 mRNA was down-regulated in SAP + IL-10 group. Conclusion IL-10 can inhibit the serum levels of TNF-α and IL-6, down-regulate the expression of ICAM-1 in kidney and improve the renal dysfunction in SAP.