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本文用抗CD_3,CD_4,CD_8McAb和ABC法检测21例重型乙肝患者肝大片,亚大片坏死区炎性浸润细胞中的T细胞亚群,CD_3~+细胞>70％,其中主要为CD_8~+细胞,CD_4~+细胞减少,CD_4~+/CD_8~+比值显著下降,与急黄肝和慢活肝比较差异显著。相反,非HBV感染性肝病患者非T细胞和CD_4~+细胞增加,CD_4~+/CD_8~+比值>1。提示重肝时T细胞可能参与了肝损伤,CD_8细胞亚群可能是介导肝细胞坏死的重要因素之一。还观察到相当数量的淋巴细胞和肝细胞HLA—DR抗原阳性,淋巴细胞与膜型HBAg(+)肝细胞密切接触。
In this paper, T cell subpopulations of inflammatory infiltrating cells in large and sub-large necrotic area of 21 patients with severe hepatitis B were detected by anti-CD_3, CD_4, CD_8McAb and ABC methods. The percentage of CD_3 ~ + cells was> 70%, mainly CD_8 ~ + cells , CD_4 ~ + cells decreased, the ratio of CD_4 ~ + / CD_8 ~ + decreased significantly, which was significantly different from that of acute yellow and slow liver. In contrast, non-T-cell and CD 4+ cells were increased in non-HBV-infected liver disease with a CD 4 + / CD 8 + ratio of> 1. It is suggested that T cells may be involved in liver injury in severe liver. CD_8 cell subpopulation may be one of the important factors that mediate hepatocyte necrosis. A significant number of lymphocytes and hepatocytes were also observed to be HLA-DR antigen positive, with lymphocytes in close contact with membranous HBAg (+) hepatocytes.