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目的:通过观察人参皂甙-Rd(G-Rd)对坐骨神经分支选择损伤(spared nerve injure,SNI)大鼠痛敏异常及延髓内脏带孤束核内P物质(substance P,SP)和NK-1受体表达的影响,探讨G-Rd的镇痛机制。方法:成年雄性SD大鼠30只,随机分为五组:空白对照组(blank control)、假手术组(sham operation)、SNI组、SNI+saline组(腹腔注射,i.p.)、SNI+G-Rd组(i.p.)。行为学检测应用von Frey纤维测定上述各组大鼠手术侧后肢机械缩足反射阈值(paw withdrawal mechanical thresholds,PWMT);用免疫荧光组织化学染色法,在激光扫描共聚焦显微镜下,对比检测上述各组间孤束核(NTS)内SP样免疫阳性产物和NK-1样免疫阳性产物的平均荧光强度(mean flu-orescent intensity,MFI)。结果:SNI模型组术后10 d,手术侧后肢的PWMT值(4.63 g)明显低于正常对照组和假手术组(18.20～20.30 g),术后20 d达到最低阈值(2.38 g);而SNI+G-Rd组的PWMT值(4.67 g)则明显高于SNI组和SNI+saline组(P<0.05)。免疫荧光染色结果显示:术后20 d时,SNI组NTS内SP样和NK-1样免疫荧光的平均强度明显高于空白对照组和假手术组(P<0.05);但SNI+G-Rd组的SP样和NK-1样免疫荧光的平均强度则明显低于SNI组和SNI+saline组(P<0.05)。结论:人参皂甙-Rd可以明显改善SNI引起的痛敏异常,其机制之一可能与其有效减少NTS内SP和NK-1受体的表达有密切关系。
Objective: To observe the effects of ginsenoside-Rd (G-Rd) on the pain sensitivity of spared nerve injure (SNI) rats and the content of substance P (SP) and NK-1 Receptor expression, explore the G-Rd analgesic mechanism. Methods: Thirty male SD rats were randomly divided into five groups: blank control group, sham operation group, SNI group, SNI + saline group, intraperitoneal injection, SNI + G- Rd group (ip). Behavioral testing von Frey fibers were used to measure the paw withdrawal mechanical thresholds (PWMT) of the hindlimbs in each group. Immunofluorescence histochemical staining was used to detect the expression of paw withdrawal threshold The average fluorescence intensity (MFI) of SP-like immunopositive products and NK-1 immunopositive products in the nucleus tractus solitarius (NTS) between groups. Results: In the SNI model group, the PWMT value (4.63 g) of the posterior limb was significantly lower than that of the normal control group and sham operation group (18.20-20.30 g) at 10 days after operation, and reached the lowest threshold (2.38 g) 20 days after operation. The PWMT value (4.67 g) in SNI + G-Rd group was significantly higher than that in SNI group and SNI + saline group (P <0.05). Immunofluorescence staining showed that the average intensity of SP-like and NK-1-like immunofluorescence in NTS of SNI group was significantly higher than that of blank control group and sham operation group (P <0.05) at 20 d after operation. However, the SNI + G-Rd The average intensity of SP-like and NK-1-like immunofluorescence was significantly lower in the SNI and SNI + saline groups (P <0.05). CONCLUSION: Ginsenoside-Rd can significantly improve the hyperalgesia induced by SNI, and one of the mechanisms may be related to its effective reduction of SP and NK-1 receptor expression in NTS.