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应用ＥＬＩＳＰＯＴ（Ｅｎｚｙｍｅ－ｌｉｎｋｅｄｉｍｍｕｎｏｓｐｏｔａｓｓａｙ）单细胞检测技术，研究了淋巴因子在诱导ＣＤ４，４５ＲＯ＋和ＣＤ４，４５ＲＡ＋Ｔ细胞向ＩＬ－４产生细胞分化过程中的影响。在存在ＰＭＡ和ｉｏｎｏｍｙｃｉｎ的情况下，ＩＬ－２和ＩＬ－４均可以诱导ＣＤ４，４５ＲＯ＋Ｔ细胞分化为ＩＬ－４产生细胞，但是只有ＩＬ－４可以诱导ＣＤ４，４５ＲＡ＋Ｔ细胞向ＩＬ－４产生细胞分化，ＩＦＮγ既不能诱导ＣＤ４，４５ＲＡ＋，亦不能对ＣＤ４，４５ＲＯ＋Ｔ细胞产生诱导作用。当内源性ＩＬ－４被中和后，ＩＬ－２对ＣＤ４，４５ＲＯ＋Ｔ细胞的诱导作用消失。本文结果初步表明ＩＬ－４在决定Ｔｈ前体细胞是向Ｔｈ１还是向Ｔｈ２方向发展起着关键的作用。
The effects of lymphokines on the differentiation of CD4, 45RO + and CD4,45RA + T cells into IL-4-producing cells were studied using ELISPOT (Enzyme-linked immunospotassay) single cell assay. Both IL-2 and IL-4 induced differentiation of CD4 + and 45 + T cells into IL-4-producing cells in the presence of PMA and ionomycin, but only IL-4 induced differentiation of CD4 + and 45RA + T cells into IL- IFNy can neither induce CD4, 45RA +, nor induce CD4, 45RO + T cells. When endogenous IL-4 was neutralized, the induction of CD4, 45RO + T cells by IL-2 disappeared. Our results preliminarily show that IL-4 plays a key role in determining whether Th precursors develop Th1 or Th2.