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应用ELISPOT(Enzyme-linkedimmunospotassay)单细胞检测技术,研究了淋巴因子在诱导CD4,45RO+和CD4,45RA+T细胞向IL-4产生细胞分化过程中的影响。在存在PMA和ionomycin的情况下,IL-2和IL-4均可以诱导CD4,45RO+T细胞分化为IL-4产生细胞,但是只有IL-4可以诱导CD4,45RA+T细胞向IL-4产生细胞分化,IFNγ既不能诱导CD4,45RA+,亦不能对CD4,45RO+T细胞产生诱导作用。当内源性IL-4被中和后,IL-2对CD4,45RO+T细胞的诱导作用消失。本文结果初步表明IL-4在决定Th前体细胞是向Th1还是向Th2方向发展起着关键的作用。
The effects of lymphokines on the differentiation of CD4, 45RO + and CD4,45RA + T cells into IL-4-producing cells were studied using ELISPOT (Enzyme-linked immunospotassay) single cell assay. Both IL-2 and IL-4 induced differentiation of CD4 + and 45 + T cells into IL-4-producing cells in the presence of PMA and ionomycin, but only IL-4 induced differentiation of CD4 + and 45RA + T cells into IL- IFNy can neither induce CD4, 45RA +, nor induce CD4, 45RO + T cells. When endogenous IL-4 was neutralized, the induction of CD4, 45RO + T cells by IL-2 disappeared. Our results preliminarily show that IL-4 plays a key role in determining whether Th precursors develop Th1 or Th2.