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Objective To study the effect of hiccough on esophageal function and its significance for providing information on clinic work. Methods Simultaneously monitoring the acid and the bile of esophageal on the patients with hiccough, and then we observe the esophageal mucus and its tissue constructer of patients with hiccough under endoscopes .In the same way, we observe the health man that is a control group. Then compared with them. Results We observed the hiccough could cause gastric esophsgeal reflux disease(GERD).The contence of acid and bile in the esophageal of patients with hiccough were increased ,the mucus and function were injured. Conclusion The hiccough can cause the GERD and the injury the mucus and the function of patients with hiccough .The reason of hiccough is connected with inflammation, tumor and the diseases of central system (CNS).