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为了研究各种大小不同卵泡的增长,曾用过几种方法使卵巢的组织成份分离。一篇新近的研究报道,为了从卵泡形成的不同阶段提取大量形态学上和功能上完整的大田鼠卵泡,而采用简单和快速的技术,其原理是用酶来消化胶元基质,使其松解,接着用缓和的机械活化作用使小的前窦细胞散开。先把大的前窦细胞、窦细胞和黄体从卵巢剖出,然后将混合好的胶元酶,DNA酶和蛋白酶混合后加入切碎卵巢组织里20分钟,摇匀后加入少量蛋白,将卵泡与细胞混悬液离心,用尼龙筛子过滤,再使卵泡悬浮,这样就可获得单个雌性动物的卵泡,三对卵巢进行上述全部过程需要4～5个小时。根据大小可以把全部卵泡分为10级:1～6级以围绕卵母细胞周围的颗粒细胞层的数目来区分;
In order to study the growth of different sizes of follicles, several methods have been used to separate the ovarian tissue components. A recent study reported that in order to extract a large number of morphologically and functionally intact voles from different stages of follicular formation using a simple and rapid technique, the principle is to enzymatically digest the collagen matrix so that its pine Solution, followed by mild mechanical activation to diffuse the small pre-sinusoidal cells. Large pre-sinusoidal cells, sinus cells and corpus luteum from the ovary dissection, and then a good mix of collagenase, DNase and protease mixed chopped ovary tissue after 20 minutes, shake a small amount of protein, the follicle Centrifugation with the cell suspension, filtration with a nylon sieve, and suspension of the follicle, thus obtaining follicles of individual females, takes about 4 to 5 hours for all of the above three ovaries. According to the size of all the follicles can be divided into 10 levels: 1 to 6 to surround the granulosa cell layer around the number of oocytes to distinguish;