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目的:检测食管鳞状细胞癌组织中核干细胞因子(NS)蛋白的表达。方法:应用免疫组化SABC法检测62例食管鳞状细胞癌组织、31例癌旁不典型增生组织及62例正常食管黏膜组织中NS蛋白的表达,并分析其与食管鳞状细胞癌患者病理分级、淋巴结转移等临床指标的关系。结果:食管鳞状细胞癌组织、癌旁不典型增生组织和正常食管黏膜组织中NS蛋白的阳性表达率分别为69.4%,41.9%和17.7%,3组间比较差异有统计学意义(χ2=33.676,P<0.01)。NS蛋白阳性表达率与食管鳞状细胞癌的组织学分级、浸润深度及淋巴结转移有关(P均<0.05),与年龄、性别无关(P>0.05)。结论:NS在食管癌的发生发展和增殖过程中起重要作用,NS蛋白检测可望成为食管鳞状细胞癌早期诊断的重要指标之一。
Objective: To detect the expression of NS protein in esophageal squamous cell carcinoma. Methods: The expressions of NS protein in 62 cases of esophageal squamous cell carcinoma, 31 cases of adjacent atypical hyperplasia tissues and 62 cases of normal esophageal mucosa tissues were detected by immunohistochemical SABC method. The expressions of NS protein in esophageal squamous cell carcinoma Grading, lymph node metastasis and other clinical indicators. Results: The positive expression rates of NS protein in esophageal squamous cell carcinoma, adjacent atypical hyperplasia and normal esophageal mucosa were 69.4%, 41.9% and 17.7%, respectively. There was significant difference among the three groups (χ2 = 33.676, P <0.01). The positive rate of NS protein was correlated with histological grade, depth of invasion and lymph node metastasis (all P <0.05), but not with age and sex (P> 0.05). Conclusion: NS plays an important role in the development and proliferation of esophageal cancer. NS protein detection is expected to be one of the important indicators in the early diagnosis of esophageal squamous cell carcinoma.