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目的:研究急性肺损伤(acute lung injury,ALI)对大鼠脾脏的丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutases,SOD)、一氧化氮(nitrogen monoxidum,NO)及结构的影响。方法:16只Wister大鼠随机分为对照组(C组)和油酸组(OA组),后者由尾静脉注射油酸法复制ALI模型2h后,测定血气(pH、PaO2、MAP、PaCO2)、肺的湿/干重(W/D)及血浆和脾脏匀浆SOD、MDA、NO。脾脏组织做光、电镜检查。结果:与C组相比,OA组PaO2、MAP降低(P<0.01);血浆和脾脏的SOD活性降低(P<0.01),MDA和NO含量增高(P<0.01);光镜下OA组脾脏被膜增厚,鞘淋巴细胞增生,灶状中性白细胞浸润,生发中心异常和红髓吞噬含铁血红素细胞增多改变;电镜下细胞肿大,核膜皱缩,核大畸形,线粒体水肿,空泡变和粗面内质网扩张改变,排列紊乱。结论:ALI可引起大鼠脾脏SOD、MDA、NO及结构的改变,氧化应激反应导致脂质过氧化损伤可能是ALI时远隔器官受损的原因之一。
Objective: To investigate the effect of acute lung injury (ALI) on malondialdehyde (MDA), superoxide dismutase (SOD), nitrogen monoxidum (NO) and the structure of rat spleen Impact. Methods: Sixteen Wister rats were randomly divided into control group (C group) and oleic acid group (OA group). The latter was induced by oleic acid injection into the tail vein for 2 hours. The blood gas (pH, PaO2, MAP, PaCO2 ), Lung wet / dry weight (W / D) and plasma and spleen homogenate SOD, MDA, NO. Spleen tissue light, electron microscopy. Results: Compared with group C, PaO2 and MAP in OA group were decreased (P <0.01), SOD activity in plasma and spleen was decreased (P <0.01), MDA and NO content were increased Envelope thickening, sheath lymphocyte proliferation, focal neutrophil infiltration, abnormalities of germinal centers and increased erythrocyte phagocytic heme cells; under the electron microscope, cell swelling, nuclear membrane collapse, nuclear deformity, mitochondrial edema, empty Bubbling and rough endoplasmic reticulum expansion changes, arranged disorder. CONCLUSION: ALI can induce changes of SOD, MDA, NO and structure in rat spleen. Oxidative stress leading to lipid peroxidation injury may be one of the reasons of distal organ damage during ALI.