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采用Ｐａｐａｎｉｃｏｌａｏｕ染色、免疫荧光和电镜技术对１３例生育者、１３例不明原因不育者和８例精液脲原体阳性不育者的精液非精子细胞（ＮＳＣ）进行分析，发现生育组ＮＳＣ占精液细胞总数的１．８８％，其中未成熟生精细胞（ＩＧ）占ＮＳＣ的８３．４％，白细胞９．１％，脱落上皮细胞６．３％，支持细胞１．２％。ＩＧ中包括无核胞浆体５０．４％，初级精母细胞１０．１％，次级精母细胞２．９％，早期精子细胞６．８％，晚期精子细胞１３．２％。白细胞中多形核细胞５．４％，巨噬细胞２．６％，淋巴细胞１．１％。淋巴细胞中以ＣＤ３和ＣＤ８阳性细胞为主，ＣＤ３／ＩｇＧ阳性细胞比为１６．５；ＣＤ８／ＣＤ４细胞比为３．６。两组不育者中，ＮＳＣ总数和除支持细胞外各组分均增加，其中淋巴细胞总数和ＣＤ４细胞增加最显著，ＣＤ８／ＣＤ４比降为０．３５，两不育组间无显著差异。正常精液中ＣＤ８细胞优势可能有利于免疫抑制作用。
Papanicolaou staining, immunofluorescence and electron microscopy of 13 cases of fertility, 13 cases of unexplained infertility and 8 cases of infertility were positive sperm non-sperm cells (NSC) were analyzed and found that fertility group NSC accounted for semen The total number of cells was 1.88%, of which immature spermatogenic cells (IG) accounted for 83.4% of NSC, white blood cells 9.1%, shedding epithelial cells 6.3%, supporting cells 1.2%. IG includes 50.4% of nucleus apheresis, 10.1% of primary spermatocytes, 2.9% of secondary spermatocytes, 6.8% of early spermatozoa, and 13.2% of late spermatozoa. 5.4% of polymorphonuclear leukocytes, 2.6% of macrophages, and 1.1% of lymphocytes. The predominant lymphocytes were CD3 and CD8 positive cells, with a CD3 / IgG positive cell ratio of 16.5 and a CD8 / CD4 cell ratio of 3.6. In both infertile groups, the total number of NSCs and the components except the supportive cells increased. The total number of lymphocytes and the increase of CD4 cells were the most significant. The ratio of CD8 / CD4 was 0.35. There was no significant difference between the two groups. The advantages of CD8 cells in normal semen may be beneficial to immunosuppression.