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应用免疫组织化学ＡＢＣ染色技术对１００例乙型肝炎、肝硬变和肝细胞癌（ＨＣＣ）患者的肝组织进行Ｃ—ｅｒｂＢ—２和ＥＧＦＲ染色，结果表明，３８．８９％（３５／９０）的慢性肝炎、肝硬变和ＨＣＣ组织有ＥＧＦＲ表达，主要定位于血窦内皮。良、恶性病变肝组织之间在ＥＧＦＲ表达强度上无显著差别，提示ＥＧＦＲ可能与慢性肝脏病变中血窦内皮增生有关。在正常肝，仅少数标本（２／１０）中见到Ｃ—ｅｒｂＢ—２微弱表达；在ＨＢＶ相关的慢性肝脏病变，所有标本中均检测到Ｃ—ｅｒｂＢ—２蛋白，主要定位于肝小多角细胞（ＳＰＬＣ），小细胞性不典型增生（ＳＣＤ）及小管状化生（ＤＭ）的肝细胞；ＨＣＣ细胞中Ｃ—ｅｒｂＢ—２蛋白阳性较弱。这提示ｃ—ｅｒｂＢ—２基因活化与人ＨＣＣ发生有关。Ｃ—ｅｒｂＢ—２与ＨＢｘＡｇ表达密切关系提示这种原癌基因的强化可能也与ＨＢＶｘ基因有关。
C-erbB-2 and EGFR staining were performed on 100 liver biopsies from 100 patients with hepatitis B, cirrhosis and hepatocellular carcinoma (HCC) using immunohistochemical ABC staining. The results showed that 38.89% (35/90) Of chronic hepatitis, cirrhosis and HCC tissue EGFR expression, mainly located in the blood sinus endothelial. There was no significant difference in the expression intensity of EGFR between benign and malignant lesions, suggesting that EGFR may be related to the proliferation of sinusoidal endothelial cells in chronic liver disease. C-erbB-2 was weakly expressed in only a few specimens of normal liver (2/10). C-erbB-2 protein was detected in all HBV-related chronic liver lesions and mainly localized in small polygons (SPLC), small cell dysplasia (SCD) and tubule metaplasia (DM). The positive expression of C-erbB-2 in HCC cells was weak. This suggests that c-erbB-2 gene activation is associated with the development of human HCC. The close relationship between the expression of C-erbB-2 and HBxAg suggests that the enhancement of this oncogene may also be related to the HBVx gene.