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目的 血管内皮细胞生长因子 (VEGF)能引起强烈的血管渗漏。越来越多的证据显示糖尿病性视网膜病变时 VEGF含量升高。本课题研究应用可溶性 Flt- 1阻断 VEGF的作用能否抑制视网膜血管渗漏。方法 重约 2 0 0 g的 Sprague- Dawley大鼠通过注射streptozotocin制备糖尿病模型。 1wk后或不给予任何处理 ,或静脉给予可溶性 Flt- 1,或PBS+ 5 0 g· L- 1 甘油 ,或白介素 - 6受体单克隆抗体 ,2 4h后应用伊凡思蓝法测定血管渗漏。枸橼酸钠缓冲液和 streptozotocin注射后未发生糖尿病的大鼠做为对照组。结果 枸橼酸钠缓冲液和 streptozotocin注射后未发生糖尿病的 2种对照组间视网膜血管渗漏无显著差异 (P>0 .0 5 )。糖尿病组和总体对照组相比有显著差异 (P<0 .0 5 )。PBS+ 5 0 g· L- 1 甘油和白介素 - 6受体单克隆抗体处理后糖尿病性大鼠的视网膜血管渗漏无变化 (P>0 .0 5 ) ,但用 2 5 m g· kg- 1 可溶性 Flt- 1处理后糖尿病性大鼠血管渗漏显著降低 (P<0 .0 0 0 1) ,达到正常水平 ,与总体对照组相比 P>0 .0 5。结论 VEGF在糖尿病性视网膜病变的发生过程中起着十分重要的作用。通过抑制 VEGF可以降低早期糖尿病性视网膜病变的血管渗漏。
Purpose Vascular endothelial growth factor (VEGF) causes intense vascular leakage. There is growing evidence that VEGF levels are elevated in diabetic retinopathy. This topic studies whether the application of soluble Flt-1 to block the role of VEGF can inhibit retinal vascular leakage. Methods Sprague-Dawley rats weighing approximately 200 g were used to prepare diabetic models by injecting streptozotocin. 1wk or no treatment, or intravenous administration of soluble Flt-1, or PBS +5 0 g · L- 1 glycerol, or interleukin-6 receptor monoclonal antibody, 24 h after the application of Evans blue method for the determination of vascular leakage . Rats without diabetes after sodium citrate buffer and streptozotocin injection served as control group. Results There was no significant difference in retinal vascular leakage between the two control groups without sodium citrate buffer and streptozotocin injection (P> 0.05). There was a significant difference between the diabetic group and the control group (P <0.05). There were no changes in retinal vascular leakage in diabetic rats treated with PBS + 50 g · L-1 glycerol and interleukin-6 receptor monoclonal antibody (P> 0.05), but no change in retinal vascular leakage with 25 mg · kg-1 The vascular leakage of diabetic rats treated with Flt-1 was significantly lower (P <0.01 01), reaching the normal level, P> 0.05, compared with the control group. Conclusion VEGF plays a very important role in the pathogenesis of diabetic retinopathy. Vascular leakage of early diabetic retinopathy can be reduced by inhibiting VEGF.