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目的：评价Ｓｏｕｔｈｅｒｎ印迹分析（ＳＢＡ）和聚合酶链反应（ＰＣＲ）检测原发性皮肤Ｔ细胞淋巴瘤（ＰＣＴＣＬ）Ｔ细胞受体（ＴＣＲ）基因重排（ＧＲ）的意义。方法：以ＰＣＲ扩增ＴＣＲγ的结合Ⅴ（可变区）－Ｊ（结合区）序列（ＴＣＲγＰＣＲ）和ＳＢＡ分析ＴＣＲβ链基因（ＴＣＲβＳＢＡ）检测克隆性ＧＲ。结果：蕈样肉芽肿（ＭＦ）：ＴＣＲγＰＣＲ和ＴＣＲβＳＢＡ检测６例ⅡＡ期和７例ⅡＢ期皮损标本的ＧＲ分别为５例和４例以及６例和５例，外周血分别有４例、２例和５例、３例示ＧＲ；而７例ⅠＡ期和１０例ⅠＢ期的ＴＣＲγＧＲ和ＴＣＲβＧＲ皮肤组织为４例、１例和７例、１例，外周血为３例、阴性和４例、１例。１例ＭＦⅡＡ表现为皮病性淋巴结病患者的淋巴结中证实有ＧＲ。疑诊ＭＦ：１１例患者的皮损和外周血标本经ＴＣＲγＰＣＲ检测５例皮肤和３例外周血见ＧＲ。非蕈样肉芽肿、Ｓéｚａｒｙ综合征的ＰＣＴＣＬ：ＰＣＲ和ＳＢＡ显示ＴＣＲＧＲ分别为皮肤组织占９例／１０例和６例／８例，外周血占９例／１０例和６例／１１例。Ｓéｚａｒｙ综合征和淋巴瘤样丘疹病：２例Ｓéｚａｒｙ综合征外周血和其中１例皮肤标本同时见ＴＣＲγＧＲ和ＴＣＲβＧＲ；２例淋巴瘤样丘疹病的皮肤标本
Objective: To evaluate the significance of Southern blot analysis (SBA) and polymerase chain reaction (PCR) for the detection of primary cutaneous T cell lymphoma (PCTCL) T cell receptor (TCR) gene rearrangement (GR). METHODS: Clonal GR was detected by PCR amplification of TCRγ binding V (variable region)-J (binding region) sequence (TCRγPCR) and SBA analysis of TCRβ chain gene (TCRβSBA). RESULTS: Mycosis granuloma (MF): The GR of 6 cases of stage IIA and 7 cases of stage IIB lesions were detected by TCR gamma PCR and TCR beta SBA in 5 cases and 4 cases and 6 cases and 5 cases respectively. There were 4 cases and 2 cases of peripheral blood respectively. Cases and 5 cases, 3 cases showed GR; 7 cases of IA stage and 10 cases of IB stage TCRgammaGR and TCRbetaGR skin tissue in 4 cases, 1 and 7 cases, 1 case, peripheral blood in 3 cases, negative and 4 cases, 1 example. One case of MFIAI showed GR in the lymph nodes of patients with dermatosis lymphadenopathy. Suspected MF: skin lesions and peripheral blood specimens of 11 patients were examined by TCR gamma PCR for 5 cases of skin and 3 cases of peripheral blood to see GR. The PCTCL:PCR and SBA of non-myeloacious granulomatosis and Sézary syndrome showed that TCGRG accounted for 9 cases/10 cases and 6 cases/8 cases of skin tissue, 9 cases/10 cases and 6 cases/11 cases of peripheral blood. Sézary syndrome and lymphomatoid papulosis: 2 cases of Sézary syndrome peripheral blood and 1 case of skin specimen also see TCRγGR and TCRβGR; 2 cases of skin specimens of lymphomatoid papulosis