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目的:建立轮状病毒(rotavirus,RV)肠道外感染乳鼠模型。方法:实验动物选用10~11日龄的清洁级ICR乳鼠,将32只乳鼠随机分为2组,每组16只,即RV肠道外感染组:乳鼠经腹腔注射150μl猴RV SA l1(病毒滴度TC ID50为10-4);对照组:乳鼠经腹腔注射150μl生理盐水。接种后每天观察乳鼠的活动状况、饮食情况、毛色、体型、大便变化等,4天后处死,留取心、肝、肾、脾、肠进行病理学切片和匀浆培养。结果:感染组在接种RV后第2天开始出现食欲不振,体毛蓬松、无光泽,活动减少,呆滞、嗜睡,对刺激反应淡漠,大便量少,上述症状逐渐加重,到第4天症状最明显,并有3只乳鼠出现腹泻,观察中累积腹泻率和发病率分别为18.75%和100.00%。光镜下肝、肾、脾、小肠有病理改变,且肝、肾、小肠组织匀浆培养72 h后出现不同程度细胞病变,余阴性。对照组乳鼠无明显变化。结论:该方法初步建立了RV肠道外感染乳鼠模型。
Objective: To establish a rotavirus (RV) model of parenteral infection in neonatal rats. Methods: The experimental animals were selected 10 to 11 day-old clean ICR suckling mice, the 32 suckling mice were randomly divided into two groups of 16, namely RV group of parenteral infection: infants via intraperitoneal injection of 150μl monkey RV SAl1 (Virus titer TC ID50 10-4); control group: neonatal rats injected intraperitoneally 150μl of normal saline. After inoculation, the activity, diet, coat color, body shape and stool of the suckling mice were observed daily. After 4 days, the rats were sacrificed and the heart, liver, kidney, spleen and intestine were collected for pathological sectioning and homogenization. Results: Infected group began appearing anorexia, body fluffy, dull, activity decreased, sluggish, drowsiness on day 2 after inoculation RV, indistinctive to irritation, less stool, the symptoms gradually aggravated, and the symptoms were most obvious on the 4th day , And 3 suckling mice showed diarrhea. The observed cumulative rates of diarrhea and morbidity were 18.75% and 100.00%, respectively. Light microscope, liver, kidney, spleen and small intestine pathological changes, and liver, kidney, small intestine homogenate after 72 h of culture appeared varying degrees of cytopathic effect, more than negative. The control group no significant changes in suckling mice. Conclusion: This method preliminarily established a model of parenteral infection in neonatal rabbits.