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目的 :探讨左旋卡尼汀 (L carnitine)对乳鼠心肌细胞在模拟缺血 (缺氧 )再灌注 (缺氧 复氧 )状态下发生损伤的保护作用及其可能的机制 .方法 :培养出生 1 3d的SD乳鼠心肌细胞 ,建立缺血再灌注损伤 (I/R)模型 ,实验分 3组 :①正常对照组 (Control) ;②I/R组 :缺氧 1 2 0min ,复氧 2 4 0min;③左旋卡尼汀组 :I/R之前 2h加入不同浓度左旋卡尼汀 ,使其终浓度分别为 1组 2 0mg/L ,2组 5 0mg/L ,3组 1 0 0mg/L ,4组2 0 0mg/L .观察指标包括 :①超氧化物酶 (SOD)活性 ;②丙二醛 (MDA)含量 ;③琥珀酸脱氢酶 (SDH)活性 ;④流式细胞仪(FCM)分析细胞凋亡率 .结果 :I/R组心肌细胞内MDA含量明显升高 ,SOD活性显著下降 ,琥珀酸脱氢酶 (SDH)活性明显变低 ,而且细胞凋亡率也显著增加 ,与Control组比较具有显著性差异 (P <0 .0 5 ) ;但是在药物治疗组 ,随给药浓度的增加 ,MDA含量逐渐恢复正常 ,SOD活性逐渐回升 ,SDH活性明显升高 ,而且细胞凋亡率呈下降趋势 ,各药物治疗组与I/R组比较各实验指标均具有显著性差异 (P <0 .0 5 ) ,表明心肌细胞在经左旋卡尼汀预处理之后 ,抗损伤能力加强 ,发生损伤的程度减少 .结论 :左旋卡尼汀对心肌细胞具有保护作用 ,其作用在一定范围内呈剂量依赖关系
Objective: To investigate the protective effect of L-carnitine on the damage of neonatal rat cardiomyocytes during simulated ischemia (hypoxia) reperfusion (hypoxia-reoxygenation) and its possible mechanism.Methods: The experimental group was divided into three groups: ① normal control group; ② I / R group: 120 min hypoxia, 240 min reoxygenation ; ③ L-carnitine group: different concentrations of L-carnitine were added 2 h before I / R, the final concentrations were 20 mg / L in group 1, 50 mg / L in group 2, 100 mg / L in group 3 Group 2 0 0 mg / L. Observation indicators include: ① superoxidase (SOD) activity; ② malondialdehyde (MDA) content; ③ succinate dehydrogenase (SDH) activity; ④ flow cytometry (FCM) The apoptosis rate of cardiomyocytes in I / R group was significantly higher than that in control group (P <0.05) .Conclusion: The content of MDA in myocardial cells of I / R group was significantly increased, the activity of SOD was significantly decreased, the activity of succinate dehydrogenase (SDH) (P <0.05). However, in the drug treatment group, with the increase of drug concentration, the content of MDA gradually returned to normal, the activity of SOD gradually rose, and the activity of SDH was obvious (P <0.05), indicating that the myocardial cells were pretreated with L-carnitine (P <0.01), and the apoptotic rate showed a downward trend. The experimental indicators of each drug treatment group and I / R group were significantly different After that, the anti-injury ability was strengthened and the extent of injury was reduced.Conclusion: L-carnitine has a protective effect on cardiomyocytes, and its effect is dose-dependent in a certain range