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Raf-1蛋白是一种丝氨酸/苏氨酸激酶,对细胞的信号传递具有重要作用。为研究Raf-1在人髓系白血病中的作用,用Northern blot检测了27例急性髓系白血病(AML)原始细胞和6名正常人外周血单个核细胞的Raf-1 mRNA表达。27例AML按FAB分类:M_1 7例,M_2 8例,M_4 4例,M_5 4例,M_6 4例,其中1例M_6是从MDS转变而来。白血病原始细胞从外周血或骨髓获得,所有病例单个核细胞中原始细胞数大于80％。Northern blot所用~(32)P-dCTP标记的探针为与外显子16及17相对应的BgI Ⅲ/Xba Ⅰ Raf-1 cDNA片段,逆转录PCR所用一对引
Raf-1 protein is a serine/threonine kinase that plays an important role in cell signal transmission. To study the role of Raf-1 in human myeloid leukemia, Northern blot was used to detect Raf-1 mRNA expression in 27 acute myeloid leukemia (AML) blast cells and 6 normal human peripheral blood mononuclear cells. Twenty-seven cases of AML were classified according to FAB: M1 7 cases, M_2 8 cases, M_4 4 cases, M_5 4 cases, M_6 4 cases, of which 1 case M_6 was transformed from MDS. Leukemia blasts are obtained from peripheral blood or bone marrow, and the number of blast cells in mononuclear cells is greater than 80% in all cases. The (32)P-dCTP-labeled probes used in the Northern blot were BgI III/Xba I Raf-1 cDNA fragments corresponding to exons 16 and 17, and were used for reverse transcription PCR.