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目的研究氟烷和安氟醚对缺血再灌注心肌功能和代谢、氧自由基的影响。方法SD大鼠80只,随机分为10小组,每组8只。采用Langendorff离体大鼠心脏模型。按给药方式又分为3大组,对照组(含4小组):平衡15min为1小组,平衡后续灌15min为1小组,平衡续灌后缺血10min为1小组,平衡续灌缺血25min后复灌30min为1小组。氟烷组(含3小组):平衡15min后,灌注含1.5MAC氟烷灌注液15min为1小组,平衡续灌后缺血10min为1小组,平衡续灌缺血25min复灌含1.5MAC氟烷的灌注液30min为1小组。安氟醚组:包括3小组,情况同氟烷组。各组记录平衡后,给药后(或续灌15min)复灌30min左室收缩压(LVSP)、左室舒张末期压(LVEDP)、左室发展压(LVDP)、左室压力升高或降低最大速率(±dp/dtmax)、心率(HR)、冠脉流量(CF)。实验结束后测定心肌超氧化物歧化酶(SOD)活性、心肌丙二醛(MDA)含量、高能磷酸盐(ATP)含量。结果安氟醚具有明显扩张冠状动脉的作用。安氟醚能促进缺血再灌注心肌冠脉流量的恢复。两用药组明显降低LVDP、+dp/dt,升高LVEDP(P<0.05);缺血再灌注后,氟烷、安氟醚的LVDP分别恢复到基础值的57%、62%,+dp/dt分别恢复到基础值的56%、67%;与对照组相比差异具有显著性。两用药组均能提高心肌ATP含量,缺血后心肌ATP下降较慢,复灌后恢复较快。复灌
Objective To study the effects of halothane and enflurane on myocardial function, metabolism and oxygen free radicals during ischemia / reperfusion. Methods Eighty SD rats were randomly divided into 10 groups of 8 rats. Using Langendorff isolated rat heart model. According to the mode of administration, the rats were divided into 3 groups: control group (including 4 groups): 1 group with 15 minutes of balance, 1 group with balanced follow-up irrigation for 15 minutes, 1 group with equal duration of ischemia after 10 minutes of ischemia, 30min after reperfusion for a group. Hyaluronan group (including three groups): After 15 minutes of equilibration, perfusion with 1.5MAC halothane perfusion solution for 15min as a group, the balance of ischemia after 10min for a subgroup, the balance of ischemia reperfusion 25min reperfusion with 1.5MAC halothane The perfusate 30min for a group. Enflurane group: including 3 groups, the situation with the halothane group. Left ventricular systolic pressure (LVSP), left ventricular diastolic pressure (LVEDP), left ventricular development pressure (LVDP) and left ventricular pressure increased or decreased after reperfusion for 30 minutes Maximum rate (± dp / dtmax), heart rate (HR), coronary flow (CF). Myocardial superoxide dismutase (SOD) activity, myocardial malondialdehyde (MDA) content and high-energy phosphate (ATP) content were measured after the experiment. Results enflurane with a significant expansion of the role of coronary artery. Enflurane can promote the recovery of coronary flow in ischemia-reperfusion myocardium. LVDP, dp / dt and LVEDP increased significantly (P <0.05). LVDP of halothane and enflurane restored to 57%, 62%, + dp / dt were restored to 56% of the baseline value, 67%; compared with the control group, the difference was significant. Both groups can improve myocardial ATP content, ATP decreased after myocardial ischemia, reperfusion faster recovery. Replenishment