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DNAs were isolated from goldfish, and the balancer bearing species of newt. They were injected separately into fertilized goldfish eggs. A balancer developed in some newt-DNA injected goldfish.DNA injected goldfish eggs occasionally developed a non-balancer process. These results demonstrated the newt DNA-mediated transfer of the species character from newt to goldfish. The injected DNA was localized by the method of autoradiography using H~3-cDNA. Silver grains were found in areas over the colchicine induced metaphase. The incorporation of foreign DNA into chromosomes provides a material basis for genetic transformation.
DNAs were isolated from goldfish, and the balancer bearing species of newt. These balance of results in the newt-DNA injected goldfish.DNA filled goldfish eggs occasionally developed a non-balancer process newt DNA-mediated transfer of the species character from newt to goldfish. The injected DNA was localized by the method of autoradiography using H ~ 3-cDNA. Silver grains were found in areas over the colchicine induced metaphase. The incorporation of foreign DNA into chromosomes provide a material basis for genetic transformation.