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应用随着研究的深入,目前FIA 已建立有各种各样的梯度技术和取样技术,例如合并带技术、止流技术、间歇交替供流技术等等。同时,它又能与各种分离技术相联用,如溶剂萃取、等温蒸馏、渗析、离子交换柱等技术,所有这些,既大大扩展了分析的应用面,也提高了分析的灵敏度和选择性。下面就介绍一些比较典型的FIA 流程及其联用技术的应用示例。图4就是几种典型的FIA 流程图。简单体系的应用血浆中自蛋白的测定(见图4-G):以溴甲酚绿试剂和盐水稀释液为两路载流,将2微升血样注入盐水载流,经稀释后再与溴甲酚绿汇合,经反应管进入流通池,在600纳米
Applications With the deepening of research, FIA has established a wide range of gradient and sampling technologies, such as combined zone technology, stop-flow technology, intermittent alternate supply technology and so on. At the same time, it can be used with a variety of separation techniques such as solvent extraction, isothermal distillation, dialysis, ion exchange columns and other technologies, all of which greatly expand the application of the analysis, but also increase the sensitivity and selectivity of the analysis . Here are some of the more typical examples of FIA processes and their use of technology. Figure 4 shows several typical FIA flow charts. Simple system of application Determination of self-protein in plasma (see Figure 4-G): bromocresol green reagent and saline diluent for the two carrier, two microliters of blood injected into the saline carrier, and then diluted with bromine Cresol green convergence, the reaction tube into the flow cell at 600 nm