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目的 :研究局灶性脑缺血时脑内血红素氧合酶 2 (HO 2 )的表达。方法 :在大鼠大脑中动脉阻塞致脑缺血的不同时间点应用免疫组化、病理学及图像分析检测HO 2的表达。结果 :正常大鼠及实验大鼠的梗死侧与非梗死侧皮质、丘脑基底节区和海马齿状回均有HO 2阳性神经元。梗死灶内无表达。梗死后各时间段 ,HO 2阳性神经元的数量无明显变化 (P >0 0 5) ,而图像分析显示1 2～ 2 4h时达最高峰 (P <0 0 1 )。结论 :脑缺血时脑内神经元有HO 2蛋白阳性表达 ,其对脑保护作用的机制有待进一步研究
Objective: To study the expression of heme oxygenase 2 (HO 2) in focal cerebral ischemia. Methods: The expression of HO 2 was detected by immunohistochemistry, pathology and image analysis at different time points of cerebral ischemia induced by middle cerebral artery occlusion in rats. Results: HO 2 positive neurons were found in both normal and non-infarcted cortex, thalamic basal ganglia and hippocampal dentate gyrus in both normal and experimental rats. Infarct no expression. There were no significant changes in the number of HO 2-positive neurons at each time point after infarction (P> 0.05), while the image analysis showed the highest peak at 1-24 h (P <0.01). Conclusion: There is positive expression of HO 2 protein in cerebral neurons during cerebral ischemia. The mechanism of its protective effect on the brain remains to be further studied