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为探究番茄早疫病菌Alternaria solani对啶酰菌胺产生抗性后膜透性的变化,采用菌丝生长法、可见分光光度法、电导率法和荧光猝灭法分别测定了抗、感菌株对NaCl渗透压的敏感性、胞内可溶性蛋白和糖的含量、膜渗透率及对啶酰菌胺的吸收量。结果表明:抗性(R-Bos)菌株在2.5 g/L NaCl浓度中生长最快,敏感(S-Bos)菌株则在5 g/L NaCl浓度中生长最快;在0~10 g/L NaCl浓度下,R-Bos菌株的生长明显快于S-Bos菌株,在20~80 g/L NaCl浓度下生长无显著差异;R-Bos菌株的可溶性蛋白和可溶性糖含量显著增高,分别达61.9%~105.2%和133.1%~161.9%;用啶酰菌胺处理菌株后,所有菌株的渗透率上升,膜透性增大,但R-Bos菌株的渗透率始终低于S-Bos菌株;且R-Bos菌株对啶酰菌胺的吸收量明显减少,为48.3%~49.9%。推测番茄早疫病菌对啶酰菌胺产生抗性后,胞内保水能力提高,对渗透压表现出耐受性,同时膜透性降低使得对药剂的吸收能力下降。
In order to explore the change of membrane permeability of Alternaria solani against boscalid, the mycorrhizal fungi were tested by mycelium growth method, visible spectrophotometry, conductivity method and fluorescence quenching method NaCl osmotic pressure sensitivity, intracellular soluble protein and sugar content, membrane permeability and the absorption of boscalid. The results showed that the resistant strain (R-Bos) grew fastest at 2.5 g / L NaCl concentration and the S-Bos strain grew fastest at 5 g / L NaCl concentration. At 0-10 g / L At NaCl concentration, the growth of R-Bos strain was significantly faster than that of S-Bos strain, and there was no significant difference in growth at 20-80 g / L NaCl concentration. The soluble protein and soluble sugar content of R-Bos strain were significantly increased to 61.9 % To 105.2% and 133.1% to 161.9%, respectively. After treatment with boscalid, the permeability of all strains increased and membrane permeability increased, but the permeability of R-Bos strain was always lower than that of S-Bos strain. The uptake of boscalid in R-Bos strain was significantly reduced from 48.3% to 49.9%. It is speculated that A. solani resistance to boscalid could increase the intracellular water holding capacity and be tolerant to the osmotic pressure. Meanwhile, the membrane permeability decreased and the absorption capacity of the drug decreased.