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目的 观察鼻咽癌(NPC)颈淋巴结转移EB病毒基因情况。方法 采用细针穿刺结合聚合酶链反应(PCR)技术对50例颈部肿块穿刺液进行EBVDNA检测。结果 30例鼻咽癌颈淋巴结转移者EBVDNA阳性数27例,阳性率为900%。7例恶性淋巴瘤者EBVDNA阳性数1例,阳性率143%。8例非NPC颈部转移癌者EBVDNA阳性数1例,阳性率为125%。5例鼻咽部炎症伴颈淋巴结增生者均未检测到EBVDNA。结论 细针穿刺结合PCR方法快速灵敏,所取标本量少,特异性高,尤其对于临床难以确认的隐匿性鼻咽癌及原发灶不明的颈部转移癌,具有一定的临床诊断价值,值得推广应用。
Objective To observe the gene expression of Epstein-Barr virus (EBV) in cervical lymph node metastasis of nasopharyngeal carcinoma (NPC). Methods Fine needle aspiration and polymerase chain reaction (PCR) were used to detect EBV DNA in 50 cases of cervical mass. Results The positive rate of EBV DNA in 30 cases of cervical lymph node metastasis of nasopharyngeal carcinoma was 27 (positive rate was 900%). Seven cases of malignant lymphoma positive EBVDNA in 1 cases, the positive rate of 143%. One case of EBVDNA was found in 8 cases of non-NPC neck metastasis, the positive rate was 125%. EBVDNA was not detected in 5 cases of nasopharyngeal inflammation accompanied by cervical lymph node hyperplasia. Conclusion Fine needle aspiration combined with PCR method is rapid and sensitive, with small sample size and high specificity, especially for clinically difficult to identify latent nasopharyngeal carcinoma and primary metastatic carcinoma of the neck with unknown primary tumor. It is worthy of clinical diagnosis Promote the application.