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建立了高效液相色谱法测定稻田土壤中环吡氟草酮的分析方法.比较了不同的色谱柱、流动相组成及提取溶液对分析结果的影响.确定优化的色谱条件为:Waters Atlantis T3色谱柱(4.6 mm×250 mm,5μm),乙腈-磷酸水(体积分数0.02％)(V/V=50:50)为流动相,流速1 mL/min,柱温30℃,进样量20μL;PDA检测器,检测波长270 nm.确定了土壤样品中目标物的提取方法:酸化乙腈提取,高速离心机分离出提取液,经盐析分液净化,旋转蒸发仪浓缩、定容.结果表明,环吡氟草酮在0.1～20.0 mg/L质量浓度范围内呈良好线性关系,线性相关系数r≥0.999;仪器检出限为0.02 mg/L.添加回收试验结果表明,方法的回收率为78.0％～88.6％,相对标准偏差(n=6)为0.90％～1.0％,方法检出限为0.005 mg/kg.该方法可应用于实际样品检测.“,”A method for the determination of flufenacet in rice soil was established by high performance liquid chromatographic ( HPLC ) .The effect of chromatographic column types , mobile phases types , and also extraction solution types on the analysis results were studied comparably in the paper .The optimum HPLC conditions were confirmed as follows: separation column: Waters Atlantis T3 chromatographic column(4.6 mm ×250 mm, 5μm),mobile phase:acetonitrile and 0.02％phosphate aqueous solution ( V/V =50:50 ), flow rate: 1 mL/min, column temperature: 30℃ and injection volume:20μL,PDA detector wavelength:270 nm.The extraction process was as follows:soil samples were extracted with acidified acetonitrile for twice , and the extract was salted out and purified by liquid-liquid distribution .Afterwards, the organic phase was condensed and analyzed by HPLC .Under the optimized conditions ,the calibration curves of flufenacet showed good linearities in the range of 0.1-20.0 mg/L mass concentration , with correlation coefficients higher than 0.999 .The instrumental detection limit of flufenacet was 0.02 mg/L.The spiked recoveries were in the range of 78.0％ ～88.6％with RSDs( n =6) of 0.9％-1.0％,and the detection limits was 0.005 mg/kg.With the advantages of convenience in operation , excellent separation on capability , high sensitivity and accuracy ,the proposed method was successfully applied to the determination of flufenacetin in real soil samples.