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近年国内外多以间接荧光抗体技术(IFAT)检测家野鼠类肺脏中的流行性出血热(EHF)病毒抗原,从而确定宿主动物种类和计算带毒率。但相当部分鼠类带毒之后不易查出抗原,其血清中存在特异性IgG抗体,此抗体较易查出。我们用可与多种鼠类IgG交叉的广谱兔抗BALb/C鼠荧光血清为抗抗体,以IFAT检测福建各地家野鼠类共3353只(鼠科3属6种)的EHF IgG抗体和肺脏抗原,获得满
In recent years, many domestic and foreign indirect fluorescent antibody technology (IFAT) detection of wild rodent hay fever in the epidemic of hemorrhagic fever (EHF) virus antigens to determine the host animal species and calculate the rate of infection. However, a considerable part of the rat is not easy to detect the antigen after antigenization, and its serum has a specific IgG antibody, which is easier to detect. We detected a total of 3353 EHF IgG antibodies (6 species of 3 genera in the genus 3 families) from Fujian Province by IFAT using broad-spectrum rabbit anti-BALB / C murine serums that crossed with various kinds of murine IgGs and lungs Antigen, get full