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通过增强与内皮细胞间的粘附并释出细胞毒性物质如过氧化物(O_2~-)、弹性蛋白酶,中性粒细胞(PMN)可促使炎症反应和引起直接的组织损伤。本实验研究了两种有激活PMN潜能的脂类介质——血小板活化因子(PAF)和白三烯B_4(LTB_4)预激PMN,以及对其表面粘附分子CD11b表达、弹性蛋白酶和过氧化物释放的作用。以Ficoll-Hypaque密度梯度离心法分离PMN,并保证PMN占分离细胞的95％、活力在99％以上。PMN用PAF或LTB_4预激2、5、10、15、30或60min,随后加趋化剂fMLP,测定过氧化物最大生成率(Vmax)和弹性蛋白酶释放百分率。PMN未经PAF或LTB_4预激单加fMLP
By enhancing adhesion to endothelial cells and releasing cytotoxic substances such as peroxides (O 2 -), elastase, and neutrophils (PMNs), inflammatory responses and direct tissue damage can be induced. In this study, we investigated the expression of two adhesion molecules, CD11b, elastase and peroxide (PMF), which are lipid mediators of platelet activating factor (PAF) and leukotriene B_4 (LTB_4) The role of release. The PMN was isolated by Ficoll-Hypaque density gradient centrifugation, and the PMN accounted for 95% of the isolated cells with a viability above 99%. PMNs were pre-excited with PAF or LTB_4 for 2, 5, 10, 15, 30 or 60 min followed by addition of chemotaxis fMLP to determine the maximum peroxide production (Vmax) and elastase release percentage. PMN Single fMLP without PAF or LTB_4 lookahead