不同蛋白质修饰剂对环糊精葡萄糖基转移酶进行修饰。在一定条件下,分别用丁二酮(DIC)、苯甲酰磺酰氟(PMSF)、二硫苏糖醇(DTT)和氯氨-T(Ch-T)处理后,酶活不受影响,说明精氨酸残基、羟基、二硫键和蛋氨酸残基与酶的活力无关。用焦碳酸二乙酯(DEPC)、N-溴代琥珀酰亚胺(NBS)和碳化二亚胺(EDC)修饰后,酶活力大幅度下降,说明组氨酸、色氨酸和羧基氨基酸为酶活力所必需。 Cyclodextrin glucosyltransferases were modified with different protein modifiers. Under certain conditions, enzymatic activity was not affected after treatment with diacetyl (DIC), benzoylsulfonyl fluoride (PMSF), dithiothreitol (DTT) and chloramine-T , Indicating that arginine residues, hydroxyl groups, disulfide bonds and methionine residues have nothing to do with the enzyme activity. After modification with diethyl pyrocarbonate (DEPC), N-bromosuccinimide (NBS) and carbodiimide (EDC), the enzyme activity decreased significantly, indicating that histidine, tryptophan and carboxyl amino acids were Necessary enzyme activity.