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目的:探讨中药补肾健脾方含药血清对人肝癌细胞株SMMC-7721细胞增殖和细胞凋亡的影响。方法:制备补肾健脾方含药血清,分为高、中、低浓度组;另设复方斑蝥组和甲状腺素组作为阳性对照组,并以空白细胞组作为空白对照组。培养人肝癌细胞株SMMC-7721进行体外实验,用四甲基偶氮唑蓝(MTT)比色法检测各组培养24、48、72 h时细胞增殖抑制率,流式细胞仪检测细胞凋亡情况。结果:各药物组含药血清对人肝癌细胞株SMMC-7721的抑制作用与浓度呈负相关、与作用时间呈正相关,在5%浓度、48 h作用下达到抑制率的高峰。中药干预组细胞凋亡率呈现浓度依赖性,高浓度组显著高于低浓度组(P<0.05);复方斑蝥组的细胞凋亡率略低于中药高浓度组(P>0.05),甲状腺素片组略高于中药低浓度组(P>0.05)。结论:补肾健脾方含药血清可抑制人肝癌细胞株SMMC-7721细胞的增殖,具有诱导人肝癌细胞株SMMC-7721细胞凋亡的趋势。
Objective: To investigate the effect of serum containing Bushen Jianpi Recipe on proliferation and apoptosis of human hepatoma cell line SMMC-7721. Methods: The drug-containing serum of Bushen Jianpi Recipe was prepared and divided into high, medium and low concentration groups. The other two groups were used as positive control group and blank group as blank control group. The human hepatocellular carcinoma cell line SMMC-7721 was cultured in vitro. The cell proliferation inhibition rate of each group was detected by MTT assay at 24, 48 and 72 h, and apoptosis was detected by flow cytometry Happening. Results: The inhibitory effect of drug-containing serum of each drug group on human hepatocellular carcinoma cell line SMMC-7721 was negatively correlated with the concentration, and positively correlated with the action time, reaching the peak of inhibition at 5% concentration and 48 h. The apoptosis rate in the Chinese medicine intervention group was concentration-dependent, the concentration in the high concentration group was significantly higher than that in the low concentration group (P <0.05), the apoptosis rate in the compound group was slightly lower than that in the high concentration group (P> 0.05) The chip group was slightly higher than the traditional Chinese medicine low concentration group (P> 0.05). Conclusion: Bushenjianpi Recipe can inhibit the proliferation of human hepatocellular carcinoma cell line SMMC-7721 and induce the apoptosis of human hepatocellular carcinoma cell line SMMC-7721.